Consensus Guideline Reached For Quantitative Polymerase Chain Reaction
qPCR is extensively used in diagnostic testing, from pathogen detection to cancer prognosis, and new studies using qPCR are published at an ever-increasing rate. The consensus guideline, the first of its kind for this technique, provides a minimum set of information that researchers must provide in order for their qPCR data to be considered for publication. The goal is to increase the transparency and quality of studies, so that experiments using qPCR can be accurately reproduced by others, and the scientific community can assess the quality and validity of those studies more readily.
Authors of this guideline, including Professor
The guideline includes a checklist of essential and desirable steps that should be followed when using qPCR. If followed appropriately, authors should be able to design and report qPCR experiments with greater inherent value, and journal reviewers, editors, and laboratory managers will be able to evaluate the technical quality of the published data against an established yardstick. Most importantly, adherence to this guideline should facilitate the publication of results that will be factually reliable.
"This guideline is important because many of these studies become the basis of new molecular diagnostic tests. They have to be reliable," commented co-author Dr.
"This guideline was created by scientists from five countries who are very knowledgeable in qPCR. The guideline should help us evaluate future qPCR studies that are submitted to our journal," remarked Dr.
About quantitative PCR (qPCR):
qPCR is an extension of the polymerase chain reaction invented in 1986 by
More about the guideline and related information:
- RDML: structured language and reporting guidelines for real-time quantitative PCR data. Nucleic Acids Res. 2009;
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