Sample Preparation in Genomics, Proteomics, and Epigenomics: Global Markets
NEW YORK, Sept. 26, 2011 /PRNewswire/ -- Reportlinker.com announces that a new market research report is available in its catalogue:
Sample Preparation in Genomics, Proteomics, and Epigenomics: Global Markets
http://www.reportlinker.com/p0619256/Sample-Preparation-in-Genomics-Proteomics-and-Epigenomics-Global-Markets.html#utm_source=prnewswire&utm_medium=pr&utm_campaign=Genomics
THIS REPORT CONTAINS:
* An overview of the major products, technologies, and services of the sample preparation industry in genomics, proteomics, and epigenomics
* Analyses of global market trends, with historical data from 2009 and 2010, estimates for 2011, and projections of compound annual growth rates (CAGRs) through 2016
* A breakdown of the sample preparation market by subcategories, including next-generation sequencing, microarrays, qPCR and PCR, RNA research, and synthetic biology
* Coverage of main reagents and kits providers in each market, including leading companies, "newcomers," and competitors
* Evaluation of recent patents and technologies
* Comprehensive company profiles.
STUDY GOALS AND OBJECTIVES
This BCC Research report, Sample Preparation in Genomics, Proteomics, and Epigenomics: Global Markets, provides an overview of the current overall state of the sample preparation industry for life science research focusing on the genomics, epigenomics, and proteomics segments. It describes existing products on the market, and a detailed analysis of the competitive environments, including new potential markets for novel products and assay development. This study includes information about significant players, trends, and obstacles and other information affecting this market and its different segments.
REASONS FOR DOING THIS STUDY
Experimental procedure and technical protocols are the basis for a successful scientific project. Sample preparation steps are often the main "bottleneck" or foundation for further downstream analysis and workflow.
Reagents companies started to recognize commercial potential in providing the required reagents, kits, and assays that help to facilitate scientific work by reducing time, labor, and even costs. Slowly, sample preparation products are evolving into a specific market space with its own trends, influences, and challenges.
Although it is part of the life science reagents and tools industry in general, the sample preparation segment exhibits specific characteristics and differences in its market participants and competitive landscape.
This report highlights the main segments in the sample preparation industry: genomics, proteomics, and epigenomics. It discusses its major players, current and future trends and influences. Drivers and challenges in each segment of this industry are described in detail, including forecasts for the next 5 years.
SCOPE AND FORMAT
This report is essentially a business tool that can help market players evaluate new commercial opportunities in the sample preparation market for genomics, proteomics, and epigenomics research. The geographic scope of this study is global. This market is complex and consists of many different segments, each of which is affected differently by scientific and technological developments. This report identifies the main positive and negative factors affecting each segment, and forecasts market growth, future trends, and product and assay development in every major segment of this market.
METHODOLOGY AND INFORMATION SOURCES
Both primary and secondary research methodologies were used in preparing this study. Initially, a comprehensive and exhaustive search of the literature on drug discovery and already marketed products was conducted. These secondary sources included drug discovery and science-related journals, textbooks, press releases, marketing literature, other product/promotional literature, annual reports, security analyst reports, and other publications. A patent search and analysis were also conducted.
In a second phase, a series of semi-structured interviews was conducted with company representatives, bench scientists, marketing representatives, and other personnel at biotechnology and pharmaceutical companies. Other sources included academics, technology, and consulting companies. Subsequent analysis of the documents and interviews notes was iterative.
INTENDED AUDIENCE
This report is intended for business development professionals, entrepreneurs, and other investors who need to fully understand and properly evaluate each segment of the market for sample preparation in genomics, proteomics, and epigenomics research. It provides insights into the market positions of major companies, and shows new opportunities otherwise overlooked or less known to the business community.
CHAPTER ONE: INTRODUCTION . 1
STUDY GOALS AND OBJECTIVES 1
REASONS FOR DOING THIS STUDY . 1
SCOPE AND FORMAT . 1
METHODOLOGY AND INFORMATION SOURCES .... 2
INTENDED AUDIENCE .... 2
ANALYST CREDENTIALS 2
RELATED REPORTS .... 3
BCC ONLINE SERVICES .. 3
DISCLAIMER . 3
CHAPTER TWO: SUMMARY . 4
SUMMARY TABLE GLOBAL MARKET FOR SAMPLE PREPARATION
REAGENTS AND PRODUCTS USED IN LIFE SCIENCE RESEARCH
BY SEGMENT, THROUGH 2016 ($ MILLIONS) 5
SUMMARY FIGURE GLOBAL MARKET FOR SAMPLE PREPARATION
REAGENTS AND PRODUCTS USED IN LIFE SCIENCE RESEARCH
BY SEGMENT, 2009–2016 ($ MILLIONS) . 5
CHAPTER THREE: INTRODUCTION TO THE SAMPLE PREP MARKET . 6
OVERVIEW 6
TABLE 1 GLOBAL MARKET FOR COMMON REAGENTS AND
PRODUCTS IN THE LIFE SCIENCE TOOLS INDUSTRY BY
SEGMENT, THROUGH 2016 ($ BILLIONS) .. 7
FIGURE 1 GENOME AND GENE ANALYSIS WORKFLOW . 8
FIGURE 2 PROTEIN ANALYSIS WORKFLOW .. 9
MAJOR TRENDS AND DRIVERS IN THE SAMPLE PREP
SEGMENT OF LIFE SCIENCE TOOLS INDUSTRY ... 9
BIOMARKER RESEARCH AND DIAGNOSTICS INDUSTRY .. 9
INNOVATIVE TECHNOLOGIES AND SCIENTIFIC TRENDS ... 10
Next-Generation Sequencing .... 10
Scientific Trends .... 10
Stem Cell Research 10
Epigenomic Research . 11
GOVERNMENT POLICIES AND FUNDING ... 11
MAIN MARKET PARTICIPANTS IN THE SAMPLE PREP
INDUSTRY ... 11
MAIN MARKET PARTICIPANTS IN … (CONTINUED) 12
TABLE 2 GLOBAL MARKET SHARES OF THE SAMPLE PREP
INDUSTRY BY LIFE SCIENCE RESEARCH SEGMENT, 2011 (%) .. 13
GEOGRAPHIC DISTRIBUTION OF THE SAMPLE PREP MARKET .... 13
TABLE 3 GLOBAL MARKET SHARES OF THE SAMPLE PREP
INDUSTRY BY GEOGRAPHIC REGION, 2011 (%) .... 14
TECHNOLOGY PATENTS IN THE SAMPLE PREP INDUSTRY . 14
TECHNOLOGY PATENTS IN THE … (CONTINUED) .. 15
TECHNOLOGY PATENTS IN THE … (CONTINUED) .. 16
CHAPTER FOUR: SAMPLE PREPARATION PRODUCTS FOR GENOMICS
RESEARCH ... 17
OVERVIEW ... 17
GENOMICS STUDIES AS A MARKET FOR THE LIFE SCIENCE
TOOLS AND SAMPLE PREPARATION INDUSTRIES .. 18
TABLE 4 GLOBAL MARKET FOR GENOMICS PRODUCTS, THROUGH
2016 ($ BILLIONS) .. 19
PRODUCTS FOR SAMPLE PREPARATION IN GENOMICS .. 19
NUCLEIC ACID EXTRACTION/PURIFICATION MARKET ... 20
Overview . 20
Magnetic Bead Based Nucleic Acid Purification .... 21
Anion Exchange Approach for Nucleic Acid Purification 22
All-in-One Application Products .... 23
Nucleic Acid Extraction Market Evaluation .... 23
TABLE 5 GLOBAL MARKET VALUE OF NUCLEIC ACID
EXTRACTION PRODUCTS, THROUGH 2016 ($ MILLIONS) .. 23
Main Participants in the Nucleic Acid Extraction Market ... 24
TABLE 6 NUCLEIC ACID MARKET SHARES BY COMPANY, 2011, (%) .. 25
Primary Types End Users in the Nucleic Acid Preparation
Market .... 25
TABLE 7 DISTRIBUTION OF THE NUCLEIC ACID PREPARATION
MARKET BY TYPE OF END USER, 2011 (%) ... 26
TABLE 8 GEOGRAPHIC DISTRIBUTION OF THE NUCLEIC ACID
PREPARATION MARKET, 2011 (%) .... 26
SPECIFIC NICHES AND SUBMARKETS IN THE NUCLEIC
ACID ISOLATION MARKET .... 27
RNA Extraction and Purification Products .. 27
RNA Extraction and … (Continued) .. 28
TABLE 9 GLOBAL MARKET SHARES OF RNA ISOLATION
PRODUCTS BY COMPANY, 2011 (%) 29
miRNA as a New Trend in Research Tools Development
and Nucleic Acid Purification Market 30
Formalin Fixed Paraffin-Embedded Tissues as a
Submarket of the Nucleic Acid Purification Market .. 31
TABLE 10 SELECT COMPANIES PROVIDING PRODUCTS FOR FFPE
NUCLEIC ACID EXTRACTION 32
Market for Nucleic Acid Extraction from Biological
Samples ... 33
Market for Nucleic Acid Extraction … (Continued) ... 34
Nucleic Acid Extraction from Difficult Samples (Plants,
Fungi, Yeast) .... 35
INNOVATIVE TECHNOLOGIES IN THE NUCLEIC ACID
PURIFICATION MARKET .. 35
Cell Lysis and Expression Analysis without Isolating
RNA.... 35
Single-Cell Analysis .... 36
Quantification of Dried Blood Spots .. 36
Specialized Applications . 36
AUTOMATION ASPECT OF THE NUCLEIC ACID
PREPARATION MARKET ... 37
TABLE 11 DISTRIBUTION OF AUTOMATION DEVICES FOR
NUCLEIC ACID PURIFICATION BY TYPE OF END USER, 2011 (%) ... 38
TABLE 12 COMPANIES THAT PROVIDE NUCLEIC ACID SAMPLE
PREPARATION PLATFORMS ... 38
TABLE 12 (CONTINUED) . 39
SUMMARY OF THE NUCLEIC ACID EXTRACTION MARKET . 40
TABLE 13 AVERAGE PRICES OF NUCLEIC ACID PURIFICATION
UNITS OFFERED BY REPRESENTATIVE COMPANIES, 2011 ($) .. 41
SAMPLE PREPARATION FOR DNA AMPLIFICATION AND
POLYMERASE CHAIN REACTION ... 41
OVERVIEW .. 41
TABLE 14 GLOBAL DNA AMPLIFICATION/PCR MARKET, THROUGH
2016 ($ BILLIONS) .. 42
TABLE 15 GLOBAL RT-PCR MARKET SHARES BY COMPANY, 2011
(%) .... 43
TABLE 16 GLOBAL PCR MAKET SHARES BY GEOGRAPHIC REGION,
2011 (%) 44
NEW TRENDS AND DEVELOPMENTS IN THE PCR
MARKET . 44
Digital PCR Trend . 44
Direct PCR ... 45
SAMPLE PREPARATION PRODUCTS IN THE PCR MARKET .. 46
TABLE 17 SAMPLE PREPARATION MARKET ESTIMATE FOR PCR
APPLICATIONS, THROUGH 2016 ($ MILLIONS) 46
Sample Preparation Products In … (Continued) ... 47
Sample Preparation Products In … (Continued) ... 48
SAMPLE PREPARATION MARKET FOR SEQUENCING
APPLICATIONS 49
SEQUENCING IN THE RESEARCH TOOLS MARKET 49
Sequencing in the Research … (Continued) . 50
TABLE 18 GLOBAL MARKET FOR DNA SEQUENCING, THROUGH
2016 ($ BILLIONS) .. 51
TABLE 19 GLOBAL MARKET FOR NGS AND GENERAL
SEQUENCING, THROUGH 2016 ($ BILLIONS) ... 52
Sequencing in the Research … (Continued) . 53
TABLE 20 NGS MARKET SHARES BY COMPANY, 2011 (%) . 54
SAMPLE PREPARATION FOR NGS EVOLVES AS A
COMPETITIVE MARKET .... 54
Overview . 54
TABLE 21 CRITERIA FOR CHOOSING A SPECIFIC VENDOR FOR
SAMPLE PREP IN NGS ACCORDING TO PRIORITY (%) ... 55
TABLE 22 VALUE OF NGS FOR THE SAMPLE PREP MARKET
THROUGH 2016 ($ MILLIONS) . 56
Drivers and Challenges for the Sample Preparation
Industry in NGS .... 56
TABLE 23 RECENT ACQUISITIONS AND COLLABORATIONS IN THE
NGS SAMPLE PREP INDUSTRY, 2011 ... 57
SAMPLE PREPARATION PRODUCTS FOR CLONING AND DNA
ASSEMBLY... 58
OVERVIEW .. 58
TABLE 24 MARKET ESTIMATION FOR SAMPLE PREPARATION IN
MOLECULAR CLONING, THROUGH 2011 ($MILLIONS) .. 59
SYNTHETIC BIOLOGY TREND AS A POSITIVE DRIVER IN
THE SAMPLE PREPARATION MARKET .. 60
Synthetic Biology Trend as a … (Continued) .... 61
ADDITIONAL TARGET SUB-MARKETS FOR SAMPLE
PREPARATION PRODUCTS FOR GENOMICS .... 62
DNA MICROARRAYS . 62
TABLE 25 GLOBAL MARKET FOR DNA MICROARRAYS, THROUGH
2016 ($ BILLIONS) .. 62
RNA RESEARCH AND ITS SAMPLE PREPARATION
MARKET . 63
Overview . 63
RNA Products ... 63
RNA Extraction Purification Kits .. 64
RNA Synthesis, Degradation, and Modification
Products . 64
TABLE 26 SELECT RNA SYNTHESIS, DEGRADATION, AND
MODIFICATION PRODUCTS .... 64
RNA Interference .. 64
TABLE 27 GLOBAL RNA INTERFERENCE MARKET THROUGH 2016
($ MILLIONS) 65
RNA Interference 66
CHAPTER FIVE: SAMPLE PREP PRODUCTS FOR EPIGENOMICS ... 67
OVERVIEW ... 67
TABLE 28 GLOBAL MARKET FOR DRUG DISCOVERY IN
EPIGENOMIC RESEARCH, 2011–2016 ($ BILLIONS) ... 67
EPIGENOMIC RESEARCH TOOLS AND PRODUCTS,
INCLUDING THE SAMPLE PREPARATION MARKET .... 68
TABLE 29 SELECT EPIGENOMIC PRODUCTS BY SUPPLIER.. 68
DNA Methylation Research and Sample Preparation
Products .. 68
DNA Methylation Research and … (Continued) ... 69
Histone Methylations and Modifications and Sample
Preparation Products . 70
TABLE 30 SELECT EPIGENOMIC SAMPLE PREPARATION
PRODUCTS BY SUPPLIER .. 71
MARKET FOR EPIGENOMICS REAGENTS AND SAMPLE
PREPARATION TOOLS .. 71
TABLE 31 PERCENTAGE OF COMMERCIAL KITS GENERALLY
PURCHASED FOR EPIGENOMIC RESEARCH BY PRODUCT TYPE ... 72
TABLE 32 GLOBAL MARKET RESEARCH TOOLS IN THE
EPIGENOMIC SEGMENT, THROUGH 2016 ($ BILLIONS) .... 73
ESTIMATED MARKET POTENTIAL FOR NEW
EPIGENOMICS PRODUCTS .... 73
TABLE 33 DISTRIBUTION OF EPIGENOMIC PRODUCTS IN THE U.S.
SAMPLE PREPARATION INDUSTRY BY TYPE OF END USER, 2011
(%) .... 74
CHAPTER SIX: PROTEOMICS RESEARCH AS A MARKET FOR THE
SAMPLE PREPARATION INDUSTRY .... 75
OVERVIEW ... 75
TABLE 34 GLOBAL MARKET FOR PROTEOMICS TOOLS, THROUGH
2016 ($ BILLIONS) .. 76
PROTEIN EXPRESSION AS A TARGET MARKET FOR
SAMPLE PREPARATION PRODUCT DEVELOPMENT .... 77
Overview . 77
TABLE 35 PERCENTAGE USE OF SPECIFIC PROTEIN EXPRESSION
TECHNIQUES, 2011 (%) ... 77
Prokaryotic Expression Systems .... 78
Mammalian Expression Systems ... 78
Protein Expression in Insect Cell Systems ... 79
Protein Expression in Yeast Cell Systems ... 79
Protein Expression in Plant Cell Systems .... 79
Cell-Free Systems .. 80
PROTEIN EXPRESSION AND SAMPLE PREPARATION
MARKET PARTICIPANTS .. 80
TABLE 36 PROTEIN EXPRESSION MARKET SHARES BY COMPANY,
2011 (%) 81
SAMPLE PREPARATION AND THE PROTEIN
PURIFICATION MARKET .. 81
Overview . 81
Extraction, Cell Lysis Procedure, and Protein
Solubilization .... 82
Protein Purification: Precipitation and Differential
Solubilization .... 83
Protein Purification: Chromatographic Methods ... 84
Size Exclusion Chromatography ... 84
Separation Based on Charge or Hydrophobicity ... 84
Affinity Chromatography .... 84
Immunoaffinity Chromatography . 85
High-Performance Liquid Chromatography 85
TABLE 37 GLOBAL MARKET FOR PROTEIN CHROMATOGRAPHY,
THROUGH 2016 ($ MILLIONS) . 86
TABLE 38 AVERAGE SPENDING PER SCIENTIST ON
PURIFICATION/SEPARATION PRODUCTS ($)... 87
SAMPLE PREPARATION FOR PROTEIN IDENTIFICATION
AND CHARACTERIZATION TECHNIQUES... 88
Mass Spectrometry 88
TABLE 39 GLOBAL MARKET FOR MASS SPECTROMETRY,
THROUGH 2016 ($ MILLIONS) . 88
Mass Spectrometry (Continued) .... 89
Protein Sequencing Approach ... 90
ICAT ... 90
MudPit .... 91
X-ray Crystallography 91
Protein NMR 92
TABLE 40 PERCENTAGE OF TOTAL NMR APPLICATIONS IN
VARIOUS SECTORS, 2011 (%) .. 92
ADDITIONAL RESEARCH TOOLS IN PROTEOMICS
STUDIES AND THEIR SAMPLE PREPARATION
PRODUCTS .. 93
Two-Dimensional Gel Electrophoresis and Its
Alternatives . 93
TABLE 41 GLOBAL MARKET FOR 2D GEL ANALYSIS, THROUGH
2016 ($ MILLIONS) . 93
TABLE 42 EXAMPLES OF 2D GEL SAMPLE PREPARATION
PRODUCTS BY COMPANY, 2011 ($) . 94
Protein–Protein Interaction Identification Techniques ... 95
Protein Microarrays .... 96
TABLE 43 GLOBAL PROTEIN CHIP MARKET, THROUGH 2016 ($
MILLIONS) .... 97
GENERAL MARKET OVERVIEW FOR SAMPLE
PREPARATION IN PROTEOMICS RESEARCH .. 98
TABLE 44 MARKET ESTIMATE FOR SAMPLE PREPARATION
PRODUCTS IN PROTEOMICS, THROUGH 2011 ($ MILLIONS) . 98
Market Participants in Proteomics Sample Preparation . 99
TABLE 45 CATEGORIES IN THE SAMPLE PREPARATION MARKET
FOR PROTEOMICS RESEARCH AND EXAMPLES OF MARKET
PARTICIPANTS .... 100
TABLE 46 EXAMPLES OF SAMPLE PREPARATION SERVICES FOR
PROTEOMICS RESEARCH AND THEIR AVERAGE COST ($) .. 100
TABLE 47 DISTRIBUTION OF THE PROTEOMICS SAMPLE
PREPARATION MARKET BY TYPE OF END USER, 2011 (%) ... 101
CHAPTER SEVEN: LABELING AND PROBES IN THE SAMPLE PREP
MARKET 102
OVERVIEW . 102
LABELING .. 102
FLUORESCENT LABELING .. 102
CHEMILUMINESCENT LABELING .... 102
RADIOACTIVE LABELING .... 102
TABLE 48 GLOBAL MARKET FOR VARIOUS LABELING AND
PROBES, 2011 ($ BILLIONS) .. 103
LABELING AND PROBES FOR GENOMICS STUDIES ... 103
LABELING AND PROBES FOR PROTEOMICS STUDIES .... 104
LABELING AND PROBES FOR … (CONTINUED) . 105
CHAPTER EIGHT: SAMPLE PREPARATION FOR THE CELL BIOLOGY
REAGENTS MARKET .... 106
OVERVIEW . 106
TABLE 49 GLOBAL MARKET FOR CELL BIOLOGY REAGENTS,
THROUGH 2016 ($ BILLIONS) .... 106
TABLE 50 GLOBAL MARKET SHARES OF CELL BIOLOGY
REAGENTS BY PRODUCT CATEGORY (%) .. 107
SAMPLE PREPARATION PRODUCTS AND SERVICES IN
THE CELL BIOLOGY SEGMENT ... 107
Cell Isolation, Maintenance, and Banking . 107
Flow Cytometry Sample Preparation .... 108
TABLE 51 GLOBAL MARKET FOR FLOW CYTOMETRY, THROUGH
2016 ($ BILLIONS) 109
Genetic Cell Identification . 109
TABLE 52 U.S. MARKET FOR STEM CELL RESEARCH TOOLS,
THROUGH 2016 ($ BILLIONS) .... 110
Genetic Cell Identification (Continued) 111
TABLE 53 REPRESENTATIVE PRICING FOR SAMPLE
PREPARATION SERVICES IN CELL BIOLOGY ($) .... 112
CHAPTER NINE: GLOBAL TARGET MARKETS FOR SAMPLE
PREPARATION BY APPLICATION .. 113
OVERVIEW . 113
FOOD SAFETY TESTING .. 113
TABLE 54 MARKET VALUE OF FOOD SAFETY TESTING FOR
PATHOGENS IN THE U.S. THROUGH 2016 ($ BILLIONS) . 114
ANIMAL HEALTH DIAGNOSTICS .. 115
TABLE 55 GLOBAL MARKET VALUE OF ANIMAL HEALTH TESTING,
THROUGH 2016 ($ MILLIONS) .... 115
FORENSICS AS A TARGET MARKET FOR THE SAMPLE
PREPARATION INDUSTRY ... 116
TABLE 56 U.S. MARKET FOR DNA TESTING SERVICES AND
PRODUCTS IN THE FORENSICS INDUSTRY, 2010 ($ MILLIONS) .. 117
BIODEFENSE AS A DRIVER MARKET FOR THE SAMPLE
PREPARATION INDUSTRY ... 118
TABLE 57 HEALTH AND HUMAN SERVICES DEPARTMENTAL
BUDGETS, 2010 ($ MILLIONS) .... 118
BIODEFENSE AS A DRIVER MARKET…(CONTINUED) .... 119
CHAPTER TEN: MARKET SUMMARY 120
OVERVIEW . 120
TABLE 58 MARKET ESTIMATE FOR SAMPLE PREPARATION
PRODUCTS IN GENOMICS, THROUGH 2016 ($ MILLIONS) .. 120
FIGURE 3 MARKET ESTIMATE FOR SAMPLE PREPARATION
PRODUCTS IN GENOMICS, 2009–2016 ($ MILLIONS) ... 121
TABLE 59 MARKET ESTIMATE FOR SAMPLE PREPARATION
PRODUCTS IN EPIGENOMICS, THROUGH 2016 ($ BILLIONS) .. 122
FIGURE 4 MARKET ESTIMATE FOR SAMPLE PREPARATION
PRODUCTS IN EPIGENOMICS, 2009–2016 ($ BILLIONS) ... 122
TABLE 60 MARKET ESTIMATE FOR SAMPLE PREPARATION
PRODUCTS IN PROTEOMICS, THROUGH 2016 ($ MILLIONS) ... 123
FIGURE 5 MARKET ESTIMATE FOR SAMPLE PREPARATION
PRODUCTS IN PROTEOMICS, 2009–2016 ($ MILLIONS) .... 123
TABLE 61 MARKET SHARE OF GENOMICS VS. PROTEOMICS
SAMPLE PREP PRODUCTS BY TYPE OF END USER, 2011 (%) .... 124
MARKET PARTICIPANTS . 124
TABLE 62 MAIN MARKET PARTICIPANTS IN THE SAMPLE
PREPARATION INDUSTRY FOR LIFE SCIENCE RESEARCH . 125
CHAPTER ELEVEN: SELECTED PROFILES OF COMPANIES 128
PROFILES … 128
AGILENT TECHNOLOGIES .. 128
GE HEALTHCARE U.K., LTD. …. 129
ILLUMINA, INC. . 130
LIFE TECHNOLOGIES CORP. … 131
NORGEN BIOTEK …. 132
PROMEGA CORP. …. 133
SIGMA-ALDRICH CORP. .. 134
THERMO FISHER SCIENTIFIC, INC. . 135
QIAGEN . 136
LIST OF COMPANIES … 136
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