Boy Interrupted: Y-chromosome Mutations Reveal Precariousness of Male Development

CLEVELAND, Sept. 2, 2013 /PRNewswire-USNewswire/ -- The idea that men and women are fundamentally different from each other is widely accepted. And throughout the world, this has created distinct ideas about which social and physical characteristics are necessary in each gender to maintain healthy human development.

However, social revolutions throughout the last century have challenged traditional ideas about not only which traits are normal and necessary for survival, but also how humans acquire them. Thanks to a new study from researchers at Case Western Reserve University, science is continuing the charge.

By studying rare families in which a daughter shares the same Y chromosome as her father, Michael Weiss, MD, PhD, and his colleagues at the university's School of Medicine have determined that the pathway for male sexual development is not as consistent and robust as scientists have always assumed.  

A team led by Weiss, chairman of the Department of Biochemistry, the Cowan-Blum Professor of Cancer Research, and a professor of biochemistry and medicine, has published a study in the Proceedings of the National Academy of Sciences that examines the function of the SRY gene. This gene is responsible for initiating the process that leads to male development. 

"A general principle of developmental biology is that evolution favors reliability," Weiss explained. "Robust switches ensure that our genetic programs give rise to a consistent body plan to ensure that babies have one heart, two arms, ten fingers, and so forth."

Traditional viewpoints emphasize the uniformity of this process. The new research indicates that male sexual development is less stable than other genetic programs. 

In fetal development, a gene located on the Y chromosome, called SRY, begins the process that leads to male development. All fetuses initially develop with female tissues, no matter what the sex will be at birth, so the master switch is responsible for initiating the transformation of female tissues into male tissues. From there, the testes develop and produce testosterone, which eventually forms the male's external genitalia.

The university's study employs mutated SRY genes shared by a father and a sterile XY daughter. Females usually develop with an XX pair, but, in these families, the father instead produced a daughter with an XY pair. This occurs during fetal development when the SRY gene's master switch fails to trigger. Internal female tissues, such as the uterus and fallopian tubes, continue to develop but are dysfunctional and infertile.

"Yet the father has the same Y chromosome and the same mutation as the daughter," Weiss pointed out. "And since he is a fertile male, we know that the switch must be poised right at its edge."

The team decided to measure the biochemical threshold of the SRY master switch.

"Our expectation was that we'd find that a factor of 100 or more—a severe insult to the Y-encoded switch—was necessary to alter development," Weiss said. "But what we found was that the SRY threshold, as probed in father-daughter pairs, is only a factor of two."

Therefore, human males actually develop near the edge of sexual ambiguity. This means that, unlike the robust genetic programs which develop other essential processes like heart function, the SRY gene master switch is particularly vulnerable to change. It only takes a slight deviation from the normal process to dramatically alter fetal sexual development.

Given the importance of sexual reproduction to the survival of a species, why do human SRY genes function so close to the boundary of infertility? The idea of an unreliable master switch might appear paradoxical, but a growing body of research suggests that it might be an evolutionary necessity.

Extensive studies of gender-associated styles of childhood play and the acquisition of social competencies by Dr. S. Baron-Cohen and colleagues at Cambridge University (UK) have highlighted the long-term effects of testosterone secretion by the fetal testis. Testosterone influences the patterning of the male brain during a critical window in human development.

And it is the SRY gene that sparks the genetic program leading to the formation of testes and the production of fetal testosterone.

"We have this tenuous switch on the Y chromosome, and we anticipate that its gift to humanity is variability in the pathway of male development from its earliest stages," Weiss said. "The essential idea is that our evolution has favored a broad range of social competencies. In prehistory, this range would have given a survival advantage to communities enriched by a diversity of gender styles."

In fact, certain aspects of modern history seem to parallel this idea.

Susan Case, PhD, a professor of organizational behavior at Case Western Reserve Weatherhead School of Management, who was not involved in the study, agreed with Weiss's argument and noted that "diverse mixes of people offer more varied perspectives, more ideas and solutions, and more challenges to long-accepted views." In the corporate world, for example, these differing styles increase creativity and problem solving, especially within a group.

The implications of Weiss's research suggest that elements of human culture, which had been assumed to be psychological or cultural, may be biological, instead. Therefore, human evolution would not have been dependent on consistency and homogeneity, but on their exact opposite.

About Case Western Reserve University School of Medicine

Founded in 1843, Case Western Reserve University School of Medicine is the largest medical research institution in Ohio and is among the nation's top medical schools for research funding from the National Institutes of Health. The School of Medicine is recognized throughout the international medical community for outstanding achievements in teaching. The School's innovative and pioneering Western Reserve2 curriculum interweaves four themes--research and scholarship, clinical mastery, leadership, and civic professionalism--to prepare students for the practice of evidence-based medicine in the rapidly changing health care environment of the 21st century. Nine Nobel Laureates have been affiliated with the School of Medicine.

Annually, the School of Medicine trains more than 800 MD and MD/PhD students and ranks in the top 25 among U.S. research-oriented medical schools as designated by U.S. News & World Report's "Guide to Graduate Education."

The School of Medicine's primary affiliate is University Hospitals Case Medical Center and is additionally affiliated with MetroHealth Medical Center, the Louis Stokes Cleveland Department of Veterans Affairs Medical Center, and the Cleveland Clinic, with which it established the Cleveland Clinic Lerner College of Medicine of Case Western Reserve University in 2002. http://casemed.case.edu

SOURCE Case Western Reserve University