Enhancing Prime Editing Efficiency Through Modified pegRNAs, Upcoming Webinar Hosted by Xtalks
In this free webinar, learn how chemical modifications to prime editing guide RNAs (pegRNAs) can improve editing efficiency while keeping the RNA sequence the same length. The featured speaker will discuss a modification strategy that improves pegRNA stability across multiple cell types while maintaining chemistry, manufacturing and controls (CMC) feasibility. Attendees will also explore how greater pegRNA stability can support stronger editing performance and future prime editing development.
TORONTO, Sept. 21, 2026 /PRNewswire/ -- Prime editing holds tremendous promise for precise genome engineering, but achieving efficient editing without increasing pegRNA complexity remains a significant challenge. Join this webinar to discover a novel pegRNA modification strategy that enhances stability and editing efficiency across multiple cell types without extending sequence length, providing actionable insights that can help accelerate prime editing research and streamline future development efforts.
Developing novel editing systems that do not require DSBs is an ever-expanding area of genetic research. Prime editing was developed as a "search-and-replace" genome editing technology. It comprises three key components:
- Prime Editing Guide RNA (pegRNA)
- Cas9 H840A Nickase
- M-MLV Reverse Transcriptase
Despite its potential, the widespread application of prime editing has been limited by suboptimal editing efficiency. While studies have demonstrated that stabilizing the 3' moiety through structural modifications (e.g., TeveproQ, G-quadruplex or hairpin motifs) can enhance efficiency, the elongation on sequence length might become problematic during CMC stage. As the general length for pegRNA would be around 130~150nt, it would be challenging to develop corresponding production processes and analytical methods.
To address this issue, GenScript screened novel modification combinations without elongating the sequence length to improve the editing efficiency. The optimized modification combination was found to be effective across multiple cell types, including HEK293T, HepG2, Jurkat and primary T cells. Further investigation revealed that the modification-optimized pegRNAs were found to be much more stable in the cytoplasm compared to canonical modified pegRNAs (three 2'-OMe and phosphorothioate on both ends).
Register now to learn how optimized pegRNA modifications can drive stronger editing outcomes in your research.
Join Jacob Guo, PhD, Director of Nucleic Acid R&D Platform, GenScript, for the live webinar on Friday, October 9, 2026, at 10am EDT (4pm CEST / EU-Central).
For more information, or to register for this event, visit Enhancing Prime Editing Efficiency Through Modified pegRNAs.
ABOUT XTALKS
Xtalks – The Life Science Community™ empowers professionals across pharma, biotech, medtech, healthcare and research with the trusted knowledge and collaborative insights that move the industry forward. Powered by Honeycomb Worldwide Inc., Xtalks delivers news, feature articles, webinars, podcasts, videos, expert interviews, curated job opportunities and more designed to support informed decision-making in a fast-evolving sector.
Every year, thousands of professionals rely on Xtalks for timely intelligence, peer perspectives and industry thought leadership. Join our life science community to stay informed, connected and ready for what's next.
To learn more about Xtalks, visit www.xtalks.com
For information about working with Xtalks to host your webinar, visit https://xtalks.com/partner-with-us/
Contact:
Soumya Shashikumar
Tel: +1 (416) 977-6555 x371
Email: [email protected]
SOURCE Xtalks
Share this article