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Lexogen presenta RiboCop rRNA Depletion Kit for Yeast
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Lexogen

Sep 01, 2022, 11:31 ET

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- Lexogen presenta el RiboCop rRNA Depletion Kit for Yeast: un nuevo kit de preparación de bibliotecas que permite la eliminación eficiente de ARNr y otras transcripciones

VIENA, 1 de septiembre de 2022 /PRNewswire/ -- Lexogen, una compañía de transcriptómica y secuenciación de próxima generación, anunció hoy el lanzamiento del nuevo RiboCop rRNA Depletion Kit for Yeast

El ARN total se compone de grandes cantidades de ARN ribosómico no deseado (ARNr), que representa hasta el 95 % de todas las transcripciones en una muestra de ARN. El nuevo RiboCop rRNA Depletion Kit for Yeast permite a nuestros clientes secuenciar lo que más importa al garantizar la eliminación eficiente del ARNr. Las lecturas de secuenciación se centran en el ARN de interés, lo que permite multiplexar más muestras y reduce significativamente el coste de secuenciación por muestra.

La tecnología de agotamiento de ARNr de RiboCop se basa en la hibridación de ARNr con oligos diseñados específicamente y la eliminación con perlas magnéticas. Lexogen ha desarrollado un algoritmo sofisticado para diseñar mezclas de sondas específicas para la máxima eliminación de ARNr, manteniendo al mínimo los efectos fuera del objetivo no deseados y preservando perfiles de expresión imparciales. RiboCop es compatible con  CORALL RNA-Seq V2 y todos los protocolos estándar de preparación de bibliotecas de ARN-Seq.

RiboCop está bien establecido para el agotamiento de ARNr humano/ratón/rata, ARNr combinado humano/ratón/rata y ARNm de globina, y para el agotamiento de muestras bacterianas. Con la incorporación más reciente de muestras de levadura, la compatibilidad de especies de RiboCop se amplía aún más y ofrece a los investigadores que trabajan con levadura varios beneficios:

  • Vista integral del transcriptoma de levadura: RiboCop ofrece la posibilidad de analizar ARN no codificante y no poliadenilado junto con ARNm y garantiza una cobertura completa de la complejidad del transcriptoma de levaduras.
  • Lecturas de secuenciación únicas maximizadas: <1,5 % de las lecturas de ARNr restantes para el rango de entrada completo para centrar las lecturas de secuenciación en el ARN de interés.
  • Escalabilidad de entrada y rendimiento: rango de entrada flexible de 10 ng a 1 µg con un rendimiento excepcional en muestras de entrada baja combinado con un protocolo fácil de automatizar.
  • Preservación de la integridad del ARN: el flujo de trabajo libre de enzimas de RiboCop preserva transcripciones completas y es ideal incluso para muestras degradadas.

"Estamos encantados con esta nueva incorporación a la familia de agotamiento de ARNr de RiboCop. Las levaduras no solo son organismos económicamente importantes en la fabricación y nutrición de alimentos, sino que algunos son patógenos humanos emergentes o existentes. Muchos investigadores en el campo están decididos a comprender el panorama transcriptómico de estos organismos en su totalidad. Con el lanzamiento de nuestro Ribocop rRNA Depletion Kit for Yeast, nuestro objetivo es apoyar a los investigadores en esta búsqueda y ayudarlos a impulsar su investigación", dijo Yvonne Göpel, directora de Producto de Lexogen.

Aprenda más sobre las ventajas del RiboCop rRNA Depletion Kit for Yeast

Aprenda más sobre las ventajas de CORALL RNA-Seq V2

Acerca de Lexogen

Establecida en 2007, Lexogen es una empresa líder en transcriptómica, secuenciación de próxima generación y análisis de ARN. La misión del equipo de Lexogen es capacitar a sus clientes con soluciones innovadoras de análisis de ARN de alta calidad y soporte, para mejorar la salud y el bienestar de todos y de nuestro planeta. Lexogen es líder en secuenciación de ARN 3', una tecnología probada por su eficiencia, robustez y sensibilidad. La cartera de Lexogen incluye kits innovadores para la secuenciación de ARN unicelular real y a granel, la purificación de ARN y el agotamiento del ARN ribosómico, los controles de variantes de ARN añadido y el etiquetado de ARN metabólico. Lexogen también proporciona servicios de análisis de ARN totalmente integrados y de primera clase, desde el diseño experimental hasta el informe analítico. Lexogen es una empresa privada, con sede en Viena, Austria, y una filial en New Hampshire, EE. UU.

Para aprender más, visite www.lexogen.com y siga a @lexogen.

Persona de contacto:
Amra Dedic
Directora de Comunicación y Eventos
Tel.: +43 660 69 66 045
E-mail: [email protected]

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