Trends of RNA Interference Screening Market (RNAi) Analyzed in 2014 Survey Report

DALLAS, October 28, 2014 /PRNewswire/ --

RnRMarketResearch.com adds RNA Interference Screening Trends 2014 market research and industry survey report to the Biotechnology section of its life sciences intelligence library. This market report summarizes the results of an industry-wide global web-based benchmarking survey on RNA interference (RNAi) screening and gene silencing carried out in October 2014. The questionnaire was compiled to meet the needs, requirements and interests of the RNAi screening vendor community. The main objectives were to comprehensively document current practices and preferences in RNAi screening, and to understand future user requirements. Complete report is available at http://www.rnrmarketresearch.com/rna-interference-screening-trends-2014-market-report.html .

The survey looked at RNAi screening as practiced today (2014) and in some cases as predicted for the future (2016). Specifically the following were investigated: key RNAi screening end-user groupings; key diseases or research area(s) targeting RNAi screening; main application areas of RNAi library screening; preferred approach to RNAi screening; types of RNAi screens investigated; main endpoints (readouts) monitored when undertaking RNAi screening; number of different RNAi screening projects attempted per year; level of throughput in RNAi screening projects; use of different cell types in RNAi research; number of different cell lines investigated when attempting RNAi screening; use of different siRNA delivery/transfection methods; how long after transfection are cells typically analyzed; total siRNA concentration typically used in transfections; final assay volume and main plate format for transfection; source of the majority of the siRNAs/shRNAs that make-up respondent's screening library; vendor compiled RNAi library groupings of greatest interest; species against which the majority of siRNAs/shRNAs are directed; size of libraries typically investigated in siRNA/shRNA screens; number of different gene targets silenced; total number of different siRNAs/shRNA constructs in libraries; are siRNAs for one given gene tested individually or as pools; number of siRNAs per pool; main limitations in RNAi library screening; main hurdles in siRNA sample preparation and liquid handling; extent to which automation has been applied to siRNA library screening; most important benefits of applying automation to siRNA screening; main suppliers of siRNA screening instrumentation platforms (i.e. liquid handling, sample prep and automated assay platforms); RNAi screening reagents and consumables budget and its breakdown into components purchased; vendor that first comes to mind when thinking of RNAi screening reagents and consumables; main supplier of RNAi screening reagents and consumables; average cost per single target gene screened by RNAi approaches; aspects of gene silencing where RNAi versus CRISPR approaches are most suited: and any unmet needs that exist in RNAi screening today.

The RNA Interference Screening (RNAi) Trends 2014 Survey responses were geographically split: 50% Europe; 26% North America; 10% Asia (excluding Japan & China); 8% Japan; 4% Rest of World; and 2% China. Respondents came from 28 University/Research Institute/Government Lab/Not-for-Profit Facilities; 6 Large Pharma; 4 Biotech Companies; 3 Medium-Small Pharma; 2 Academic Screening Centers; 2 Contract Research Organizations; 2 Agrochemical/Agri-Biotech Companies; 2 Medical Schools/Hospitals/Clinics; and 1 Biopharma. Most survey respondents had a senior job role or position which was in descending order: 10 principal investigators; 8 senior scientists/researchers; 6 section/group leaders; 6 directors; 4 professors/assistant professors; 3 research scientists/associates; 3 graduate students; 3 post-docs; 3 department heads; 2 others; 1 lab manager; and 1 vice president. Respondents represented the following key siRNA screening end user groupings: high throughput screening/phenotypic assays (36%); basic/academic research (34%); gene/target identification/validation (22%); clinical research (4%); and other industry/applied research (4%).

The RNAi screening throughput achieved today was a median 100-500 wells per week. The siRNA delivery method most used today was standard chemical (lipid-mediated) transfection. The median total siRNA concentration used in transfection today was 10-30nM. The final assay volume used for siRNA transfection today was a median of 25μL-50μL. The microplate format most used for siRNA delivery (transfection) and screening today was 384-well (standard volume). The median annual budget for RNAi screening reagents and consumables last year (2013) was $25K-$50K per lab per year. The suppliers of siRNA screening instrumentation most used by respondents came mainly from Thermo Scientific/Life Technologies, PerlinElmer, Tecan and BioTek. Order a copy of this research at http://www.rnrmarketresearch.com/contacts/purchase?rname=234168 .

A bottom-up model was developed around respondent's annual budget to estimate the global market for RNA screening reagents and consumables. In 2013 this market was estimated to be around $40M. Segmentation & CAGR estimates are given in the full report. The breakdown of the annual RNAi screening reagents and consumables budget saw the greatest share allocated to transfection reagents and buffers. The supplier/vendor of RNAi screening reagents and consumables that first comes into the mind of respondents was Thermo Scientific/Life Technologies. The suppliers/vendors of RNAi screening reagents and consumables most used by respondents were Thermo Scientific/Life Technologies and GE Dharmacon. The median cost per single target gene investigated by RNAi screening approaches was $1-$2.5. RNAi approaches were preferred over CRISPR for most technology attributes related to gene silencing. All Respondents feedback on any unmet needs that exist today in RNAi screening were documented. The full report provides the data, details of the breakdown of the responses for each question, its segmentation and the estimates for the future (2016). It also highlights some interesting differences between survey groups.

Another research titled RNA Analysis/Transcriptomics Market by Technology (Microarray, PCR, NGS, RNAi), by Application (Clinical Diagnostics, Drug Discovery, Toxicogenomics), by End-User (Academic Research, Pharmaceutical and Biotech Companies) - Global Forecast to 2019 says global transcriptomics market is expected to grow at a CAGR of 13.7% during the forecast period of 2014 to 2019 and is estimated to be worth $3,773.0 million by 2019. By 2019, North America will continue to dominate and drive the transcriptomics market, primarily due to the rising preference for personalized medicines which use biomarkers developed with the transcriptomic technologies. On the other hand, the market in Asia-Pacific region is expected to grow at a highest CAGR during the forecast period mainly due to the growing pharmaceutical and biotech industries in China and India. The reduction in funding for research and development coupled with decrease in the investment in research by the pharmaceutical and biotechnology companies in the European region as a means of cost containment is expected to hamper the growth of transcriptomics market in Europe.

The prominent players in the global trancriptomics market are Affymetrix Inc. (U.S.), Agilent Technologies Inc. (U.S.), Bio-Rad Laboratories Inc. (U.S.), GE Healthcare (U.S.), Illumina Inc. (U.S.), Qiagen N.V. (Germany), F. Hoffmann-La Roche Ltd. (Switzerland), Sigma Aldrich (U.S.), Fluidigm Corporation (U.S.), and Thermofisher Scientific Inc. (U.S.). Complete report is available at http://www.rnrmarketresearch.com/rna-analysistranscriptomics-market-by-technology-microarray-pcr-ngs-rnai-by-application-clinical-diagnostics-drug-discovery-toxicogenomics-by-end-user-academic-research-pharmaceutical-an-market-report.html .

Explore more reports on the biotechnology market at http://www.rnrmarketresearch.com/reports/life-sciences/biotechnology and on the RNAi technology industry at http://www.rnrmarketresearch.com/reports/life-sciences/biotechnology/rnai-technology .

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