MADRID, December 1, 2016 /PRNewswire/ --
PharmaMar (MCE: PHM), a world leader in the discovery, development and commercialization of new antitumor compounds of a marine origin, has announced that its antibody-drug conjugate (ADC) has demonstrated a remarkable in vitro antitumoral activity in a CD13, fibrosarcoma positive animal model. CD13 is an aminoectopeptidase, involved in the modulation of several vasoactive peptides and is known to influence major biological events, such as cell proliferation, invasion and angiogenesis. MI130110 is formed by a compound of marine origin (PM050489) that has been conjugated to a monoclonal anti-CD13 antibody through a non-hydrolysable linker. The results of this study have been presented today during the EORTC-NCI-AACR international meeting on "Molecular Targets and Cancer Therapeutics" that is taking place in Munich (Germany).
Juan Manuel Dominguez, Screening and Biochemistry Departmental Manager of PharmaMar´s Oncology Business Unit, has presented the data on the study entitled "MI130110, a new ADC combining an anti-CD13 antibody and a payload of marine origin shows remarkable in vivo activity" (abstract #397), and carried out in collaboration with both Prof. Francisco Sánchez Madrid´s and Dr Juan Manuel Zapata´s research groups from the Madrid Autónoma University, within the framework of the "Marinmab" project, financed by the Ministry of Economy.
MI130110 showed a high potency and good selectivity in in vivo in tumor cells that expressed CD13, namely NB-4 and HT-1080, with respect to other cell lines that do not express the protein (RPMI-8226 and Raji). The authors found that MI130110 impaired tubulin polymerization and disrupted the microtubule network, impairing its function during cell division, this, leading to mitotic aberrations in tumor cells that express CD13. These findings are consistent with the mechanism of action of the antitumoral compound PM050489, present in the ADC.
CD13, fibrosarcoma positive HT-1080 tumor cells were subcutaneously implanted in immunosuppressed mice, to develop tumors of approximately 180 mm. After the treatment with MI130110 at different doses, once weekly for two weeks, the mice bearing HT-1080 tumors that received the highest dose showed an important reduction in tumor size, with complete remission and a significant increase in median survival time. As expected, MI130110 treatment didn´t produce any antitumoral effect on animals that didn´t express CD13, as those in subcutaneously implanted RPMI-8226 multiple myeloma cell lines.
The effect of MI130110 in CD13 positive tumors was confirmed 24 hours after the treatment, through the appearance of alterations in the mitosis of the tumor cells, demonstrating that the antitumor activity derives from disrupting the microtubule.
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