LA JOLLA, Calif., Nov. 20, 2014 /PRNewswire/ -- SGI-DNA announces the launch of the Gibson Assembly® HiFi 1 Step Kit which allows researchers to assemble multiple, large DNA fragments in a simple one hour, one-step reaction.
This is the first high fidelity kit on the market using the Gibson Assembly® method. Researchers can now assemble up to 5 fragments, including large constructs (up to 100 kb), in a fast, reliable reaction.
"With the Gibson Assembly® HiFi 1 Step Kit researchers can obtain a perfect clone in their initial attempt," says Vibhu Gupta, Senior Product Manager at SGI-DNA. "The kit provides a proprietary enzyme formulation for Gibson Assembly® that results in significantly less errors at cloning junctions which is a common challenge for researchers in a variety of disciplines."
A researcher from the University of California, San Diego, Erna van Niekerk, PhD., comments, "I have tried it with a construct and ran a parallel experiment with a competing kit from another vendor. I picked 3 colonies from the Gibson Assembly® HiFi 1 Step Kit and 3 colonies from the competing product. Three of 3 Gibson clones gave me the right insert. Zero of 3 from competing kit gave me the right insert. Of the 3 Gibson clones: 2 of 3 had the correct sequence both forward and reverse sequencing confirmation and 1 of 3 had an extra nucleotide."
The Gibson Assembly® method was developed in 2009 by Dan Gibson, Ph.D., and his colleagues at the J. Craig Venter Institute during the team's quest to construct the first synthetic cell. It uses a one-step, isothermal approach to enable the rapid assembly of oligonucleotides and multiple DNA fragments. Since its introduction to the life science community, the Gibson Assembly® method has become a mainstay in many synthetic biology laboratories and has attracted interest from both the academic and commercial life sciences community due to its ease-of-use, robustness and flexibility. This Gibson Assembly® kit provides researchers SGI's first commercially available option utilizing the Gibson Assembly® method.
The Gibson Assembly® HiFi 1 Step Kit is available in 3 sizes: 5 reactions (starter kit), 10 reactions and 50 reactions.
For more information on the Gibson Assembly® HiFi 1 Step Kit, please visit www.sgidna.com
All products are intended for research use only. Not intended for diagnostic uses.
The Gibson Assembly® method is also available under commercial license. For more information contact us at [email protected]
SGI-DNA, a wholly owned subsidiary of Synthetic Genomics, Inc (SGI), is responsible for all commercial aspects of SGI's synthetic DNA business and focuses on strategic business relationships with both academic and commercial researchers. Building on the scientific advancements and breakthroughs from leading scientists such as J. Craig Venter, Ham Smith, Clyde Hutchison, Dan Gibson and their teams, SGI-DNA utilizes unique and proprietary DNA technologies to produce complex synthetic genes and reagents. SGI-DNA also offers a comprehensive suite of genomic services, including whole genome sequencing, library design, and other bioinformatics services.
SGI-DNA Technical contact: Vibhu Gupta, Senior Product Manager, 858.433.2288; [email protected]
Media contact: Heather Kowalski, [email protected]; 858-361-0466
SOURCE Synthetic Genomics Inc.