SAN JOSE, Calif., Dec. 1, 2016 /PRNewswire/ -- For nine years running, The Scientist has sought out recently released laboratory and research products that are making waves in life science laboratories. And for nine years, their expert panels of independent judges have highlighted some of the most innovative life science tools, software, and techniques to hit the market. The Milo Single-Cell Western platform by ProteinSimple, a brand of Bio-Techne Corporation, has been selected as the #1 Innovation of 2016 by The Scientist magazine.
The Milo Single-Cell Western system makes it possible to perform westerns (an analytical technique to detect proteins) on thousands of single-cells in parallel for the first time. The assay is performed in a scWest chip which utilizes microfluidic technology to isolate and capture ~1,000 single-cells. Milo then lyses the cells, completes a rapid (~60 sec) electrophoretic SDS-PAGE separation on each single-cell lysate, and immobilizes the separated protein bands in the gel – thereby eliminating the transfer step of traditional westerns. scWest chips are probed with conventional western antibodies, allowing researchers to measure proteins for which there are not good flow or mass cytometry antibodies and obtain western-based protein validation of their single-cell RNA results.