NEB® Launches New NEBNext® Ultra™ II Kit for NGS Library Preparation with as Little as 500 pg of Input DNA

New Ultra II technology addresses lower input amounts and challenging sample types for Illumina® next generation sequencing systems

Oct 16, 2015, 13:21 ET from New England Biolabs

IPSWICH, Mass., Oct. 16, 2015 /PRNewswire/ -- New England Biolabs® (NEB) today announced the release of the NEBNext Ultra II DNA Library Prep Kit for Illumina® next generation sequencing systems. The new Ultra II kit substantially improves upon its predecessor to produce libraries of both higher quality and higher yield from a variety of sample types, including challenging ones, such as formalin-fixed, paraffin-embedded (FFPE) samples and those containing low amounts of input DNA.

"The boundaries of library construction are constantly expanding as sequencing technologies evolve, necessitating reagents and protocols that are reliable, and don't compromise library quality," said Fiona Stewart, NEBNext Product Marketing Manager at NEB. "We've re-engineered our reagents for this new generation of NEBNext kits to accommodate as little as 500 pg of input DNA, increase yields, use fewer PCR cycles, and provide even more uniform GC coverage, all with an automatable workflow."

The NEBNext Ultra II kit addresses many of the difficulties associated with library preparation, according to reports by early access users. For Vladimir Benes, Ph.D., Head of GeneCore at EMBL in Germany, low-input genomic DNA is a significant hindrance with other protocols.

"The possibility to use the new NEBNext Ultra II DNA kit has provided us with a critical opportunity to process challenging low-input genomic samples. These samples otherwise didn't yield libraries of adequate complexity, required for exploring their genomes comprehensively," said Benes. "Using the NEBNext Ultra II DNA Kit has alleviated this problem and enabled us to deliver high quality, high content sequencing data that are relevant for our users."

"The protocol is very easy to follow and is a step up from the standard NEB Ultra Kit," added Christopher Smith, Ph.D., Post-Doctoral Research Associate at the Cancer Research UK - Cambridge Institute (CRUK-CI). "Using as little as 500 pg of cell-free DNA input, we were able to generate libraries that were successfully used for subsequent exome capture."

The NEBNext Ultra II workflow combines the End Repair and dA-Tailing steps and minimizes clean up, making the kit fast and easy to use. NEB's protocol for constructing DNA libraries with Ultra II requires just over 10 minutes hands-on time and can be finished in ~2.5 hours. The protocol is compatible with adaptors and primers from NEB or other sources, and can be used in PCR-free and target-enrichment workflows. NEBNext Ultra II DNA Library Prep is available in kit or module format, and the NEBNext Ultra II End Repair/dA-Tailing Module is also compatible with some Oxford Nanopore MinION™ workflows.

For more details about the NEBNext Ultra II DNA Library Prep Kit, visit

About New England Biolabs:
Established in the mid 1970's, New England Biolabs, Inc. (NEB) is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, synthetic biology, glycobiology, epigenetics and RNA analysis. Additionally, NEB is focused on strengthening alliances that enable new technologies to reach key market sectors, including molecular diagnostics development. New England Biolabs is a privately held company, headquartered in Ipswich, MA, and has extensive worldwide distribution through a network of exclusive distributors, agents and seven subsidiaries located in Canada, China, France, Germany, Japan, Singapore and the UK. For more information about New England Biolabs visit

NEB®, NEW ENGLAND BIOLABS®, and NEBNext® are registered trademarks of New England Biolabs, Inc.
ILLUMINA® is a registered trademark of Illumina, Inc.
MINION™ is a trademark of Oxford Nanopore, Inc.

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SOURCE New England Biolabs