SGI-DNA Launches Gibson Assembly® Site-Directed Mutagenesis Kit for Accurate, Robust and Efficient Site-directed Mutagenesis of DNA Fragments

Sep 01, 2015, 11:00 ET from SGI-DNA

LA JOLLA, Calif., Sept. 1, 2015 /PRNewswire/ -- SGI-DNA, a Synthetic Genomics, Inc. company announces the launch of the Gibson Assembly® Site-Directed Mutagenesis Kit which allows researchers to efficiently and accurately perform site-directed mutagenesis on DNA fragments. This kit is the third in SGI-DNA's Gibson Assembly® portfolio, which empowers life science researchers to substantially increase their ability to build genes, edit them, and study their functions using nucleotide insertions, substitutions, or deletions. The Gibson Assembly® Site-Directed Mutagenesis Kit has been optimized to maximize the number of gene editing studies scientists can perform, as it uses less DNA starting material, accommodates larger DNA fragments, and has a higher efficiency compared to existing kits. More information about SGI-DNA's Gibson Assembly® kit and reagent portfolio can be found at sgidna.com/gibson-assembly.

The Gibson Assembly® method was developed in 2009 by Daniel Gibson and his colleagues at the J. Craig Venter Institute during the team's quest to construct the first synthetic cell. Since its introduction to the life science community, the Gibson Assembly® method has become a mainstay in many synthetic biology laboratories and has attracted interest from both the academic and commercial life sciences community due to its ease-of-use, robustness, and flexibility. The Gibson Assembly® Site-Directed Mutagenesis Kit complements the Gibson Assembly® HiFi 1-Step Kit and the Gibson Assembly® Ultra Kit, which have been developed and optimized for robust DNA assembly by SGI-DNA in close collaboration with Daniel Gibson's research group at Synthetic Genomics.

"I'm pleased that the DNA assembly technologies we developed to build the first synthetic cell can now be used by other scientists to study genes, families, and pathways more completely," said Dan Gibson. "Molecular biologists can now greatly advance their genomics research by increasing their capacity to study the activities of genes and proteins."

Starting with a circular or a linear DNA template up to 25 kilobases in length, scientists can now introduce up to 5 site directed mutations in a single round. The Gibson Assembly® Site-Directed Mutagenesis Kit enables researchers to accommodate one or up to five site mutations with efficiencies of 95% and 55% respectively. Only 5 nanograms of DNA starting material is needed with this kit, compared to other commercially available kits that require up to 100 nanograms of DNA. The Gibson Assembly® Site-Directed Mutagenesis Kit is available in 2 sizes: 5 reactions (starter kit) and 10 reactions.

For more information on the Gibson Assembly® Site-Directed Mutagenesis Kit, please visit www.sgidna.com/sdm-kit

All products are intended for research use only. Not intended for diagnostic uses.

The Gibson Assembly® method is also available under commercial license. For more information contact us at info@sgidna.com

About SGI-DNA

SGI-DNA, a wholly owned subsidiary of Synthetic Genomics, Inc (SGI), is responsible for all commercial aspects of SGI's synthetic DNA business and focuses on strategic business relationships with both academic and commercial researchers. Building on the scientific advancements and breakthroughs from leading scientists such as J. Craig Venter, Ham Smith, Clyde Hutchison, Dan Gibson and their teams, SGI-DNA utilizes unique and proprietary DNA technologies to produce complex synthetic genes and reagents. SGI-DNA also offers a comprehensive suite of genomic services, including whole genome sequencing, library design, and other bioinformatics services.

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SOURCE SGI-DNA



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